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多菌种固态生料仿生发酵豆粕的研究硕士论文

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菌种 固态 生料 仿生 发酵 豆粕 研究 硕士论文
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安徽农业大学硕士学位论文多菌种固态生料仿生发酵豆粕的研究姓名:朱平军申请学位级别:硕士专业:动物营养与饲料科学指导教师:程茂基2011-06II Abstract Transform soybean meal into higher value­added soybean products with microbial fermentation technology is a hot research at home and abroad currently.The foreign industrial products have been formed. However, the domestic industry is still in the early stages in this area. In this study,soybean meal was fermented with Aspergillus niger,Bacillus subtilis, Yeast, and Lactobacillus.Use protease activity and acid soluble protein content of soybean meal as indicators,study the effect of single and mixed microbe fermentation on nutritive value of soybean meal.The best fermentation condition is found by single and mixed microbe fermentation soybean meal.On this basis, the multi­strain bionic fermented soybean meal on production performance of lactating sows. Experiment 1: Growth curve of different experimental microbe and determine the inoculation time.The results showed that:Aspergillus niger,Bacillus subtilis,Yeast and Lactobacillus are the most appropriate time to seed liquid culture inoculated with 24h. Experiment 2: Effect of different microbe fermentation on the nutritional value of soybean meal. The results showed that different strains of the following optimal fermentation conditions, can improved nutritional value of soybean meal. Aspergillus niger: aerobic fermentation, fermentation time 72 h, temperature 30℃, inoculum 4% and water ratio 1:0.9; Bacillus subtilis: aerobic fermentation, fermentation time 60 h, temperature 30℃, inoculum 3% and water ratio 1:0.9; Yeast: aerobic fermentation, fermentation time 48h, temperature 30℃, 2% inoculum and water ratio 1:0.9; Lactobacillus: anaerobic digestion,fermentation time 72h,temperature 37 ℃, inoculum 2% and water ratio 1:1. Experiment 3: Effect of multi­strain solid state bionic fermentation on the nutritional value of soybean meal. On the basis of experiment 2, the two­stage fermentation pattern as follow: first aerobic fermentation,then anaerobic bionic fermentation. By optimize of multi­strain orthogonal test conditions,we found the best conditions of bionic fermentation soybean meal, they are as follows: the total fermentation time was 96h. The first 48 hours, under the condition of 30 ℃, use Aspergillus niger, Bacillus subtilis and Yeast aerobic fermentation .Then, the last 48 hours, under the condition of 37 ℃,use Lactobacillus anaerobic bionic fermentation. Four strains the proportion of the total inoculation is 10%, Aspergillus niger, Bacillus subtilis, Yeast and Lactobacillus were inoculated 4%, 2%, 2%, 2%. Four strains were inoculated before fermentation. Aerobic fermentation stage water ratio is 1:0.9, anaerobic fermentation stage of biomimetic material to water ratio of 1:1.2.
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